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ybgE [2019-06-18 09:27:23]
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ybgE [2019-06-18 09:27:23]

branched-chain amino acid aminotransferase
locus
BSU02390
pI
4.95
mw
39.52 kDa
protein length
356 aa Sequence Blast
gene length
1071 bp Sequence Blast
function
biosynthesis of branched-chain amino acids
product
branched-chain amino acid aminotransferase
essential
no
ec
2.6.1.42
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.12|Biosynthesis/ acquisition of branched-chain amino acids]

    • Gene

    Coordinates
    259,016 260,086

    The protein

    Catalyzed reaction/ biological activity

  • l-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate (according to Swiss-Prot)

    • Protein family

  • [SW|Class-IV pyridoxal-phosphate-dependent aminotransferase family] (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|8163996FCB9D02F771E7A04A91D5720027260F12|YwaA]

    • [SW|Cofactors]

  • PLP

    • Structure

  • [PDB|3HT5] (from ''Mycobacterium tuberculosis'', 48% identity) [Pubmed|19923721]

    • Expression and Regulation

    Operons

    genes
    [gene|2A20EF701B9F78FC21A33D6BD8ED862323BA59A8|ybgE]
    description
    [Pubmed|15060025]

    regulatory mechanism

  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, [Pubmed|12618455,21699902,18083814], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]

    • regulation

  • repressed by casamino acids [Pubmed|12107147]
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    Biological materials

    Mutant

  • MGNA-B939 (ybgE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1938 NBRP B. subtilis, Japan]
  • a ''ybgE'' mutant and a ''[gene|A52E50104B18D0A8518218C52D9CC36FDDB29AAF|bcd] [gene|2A20EF701B9F78FC21A33D6BD8ED862323BA59A8|ybgE] [gene|8163996FCB9D02F771E7A04A91D5720027260F12|ywaA]'' triple mutant are available in [SW|Linc Sonenshein]'s lab
  • BKE02390 ([gene|2A20EF701B9F78FC21A33D6BD8ED862323BA59A8|ybgE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE02390 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATCTAATCAGCACACCTT, downstream forward: _UP4_TGAAAATCGAAAAAGAACCT
  • BKK02390 ([gene|2A20EF701B9F78FC21A33D6BD8ED862323BA59A8|ybgE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK02390 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATCTAATCAGCACACCTT, downstream forward: _UP4_TGAAAATCGAAAAAGAACCT

    • lacZ fusion

  • pGP248 (in [SW|pAC5]), available in [SW|Jörg Stülke]'s lab

    • References

  • 18083814,12618455,12670965,15060025,12107147,21699902,24163341,19923721